Predation and Inhibitors in Lake Water Affect the Success of Inoculation to Enhance Biodegradation of Organic Chemicals

Résumé du livre

P-Nitrophenol (PNP) at 50, 75, and 100 microgram/L was mineralized extensively in lake water inoculated with Corynebacterium sp. but not in uninoculated lake water. The bacterium mineralized a far lower percentage of PNP at 26 microgram/L than at the higher concentrations. Cycloheximide did not affect mineralization in lake water containing the three higher concentrations, but the extent of mineralization and Corynebacterium sp. numbers at 26 microgramo of PNP/L were reduced by this eucaryotic inhibitor. Dialysis of lake water did not inlfuence the rate of mineralization of 1.0 microgram/L, but it reduced the acclimation period, stimulated Corynebacterium sp., and usually enhanced the rate of its mineralization of PNP at 10 and 26 microgram/L. An inhibitor of PNP metabolism and Corynebacterium sp. could be removed from lake water by a cation-exchange resin. A mixed culture mineralized PNP at 26 microgram/L faster than Corynebacterium sp. The data show that predation or inhibitors may markedly affect bacteria inoculated into natural waters to promote biodegradation of low concentrations of organic chemicals.