The Modification of the Camv 35S Promoter for Preferentially Higher Expression of Transgenes in Leaf Tissues of Transgenic Plants

Résumé du livre

Two plant expression vector systems, pJL1 and pJL2 have been constructed (Joseph et al., 1996) and successfully transformed into Nicotiana tabacum var sampson fot the purpose of increasing overall transgene expression with preferentially higher expression in leaf tissues of transformed target plants. Modified CaMV 35S promoters were spliced into pBI 121, a plant gene expression vector based on the binary plasmid pBIN 19 containing the B-glucuronidase gene (uidA, gusA) or GUS gene. PJL1 has a trucated 187 bp CaMV 35S promoter where as pJL2 has a tandem repeat of the -90 to 179 region of the CaMV 35S promoter. [Authors' abstract].