Evaluation of Heat Stable B. Pseudomallei Antigens in a Dot Enzyme Immunoassay

Evaluation of Heat Stable B. Pseudomallei Antigens in a Dot Enzyme Immunoassay

Auteur : Mei Kid Leong

Date de publication : 1997

Éditeur : Secretariat 9th National Biotechnology Seminar c/o Centre for Gene Analysis, Faculty of Life Sciences, Universiti Kebangsaan Malaysia

Nombre de pages : 4

Résumé du livre

However, from the 103 IHAT positive serum samples showed only 20/103 (19.4%) were negative of which 6/26 (23%) were from IHA titer of 1:40, 4/19 (21.1%) from IHA titer of 1:80, 9/25 (36%) from IHA titer of 1:160, and 1/16 (6.3%) from the IHA titer of>1:640. Higher rate of strong positive samples in 8/17 (47.1%) and 9/16 (56.3%) by dot enzyme immunoassay demonstrated from IHA titer of 1:320 and> 1:640 respectively. Three culture positive melioidosis cases from post discharged patients showed very high titers of 1:100,000 and>1:6000 in DEIA and IHA titers of 1:2560, 1:1280 respectively. The study showed promising results for further utilization of dot enzyme immunoassay in seroepidemiological, surveillance and clinical melioidosis study. [Authors' abstract].

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