Primer Activity of Thymus Dna Fractionated by Ecteola Column Chromatography
Auteur : W. D. Skidmore, R. K. Main, L. J. Cole
Date de publication : 1963
Éditeur : U.S. Naval Radiological Defense Laboratory
Nombre de pages : 21
Résumé du livre
The DNA-primer activity of Ecteola fractionated calf- and rat-thymus DNA samples was determined by a DNA-polymerase assay system. All DNA samples assayed, heated or unheated, and fractionated or unfractionated, showed some degree of DNA-primer activity. The chromatographic profiles of heated DNA were different from those of unheated DNA. Phenol and p-aminosalicylate, used in the preparation of DNA, did not alter the patterns of DNA obtained by Ecteola chromatography. Sephadex chromatography was found to be a rapid and effective method to deionize DNA solutions. DNA-primer activity was increased by an ammonium hydroxide gradient. At 4 degrees, treatment of DNA by phenol and p-aminosalicylate, Ecteola column chromatography with a salt gradient, Sephadex column chromatography with deionized distilled water to desalt DNA, and lyophilization did not alter DNA-primer activity. The results indicate that DNA-primer activity, per se, is not specifically associated with one particular fraction of DNA. (Author).